Fatty acids are major building blocks of membrane lipids and precursors of many signaling substances. In the form of triglycerides, they are abundant components of our nutrition. Major organs of fatty acid metabolism are gut, liver, muscle and adipose tissue, but all other tissues also have the capacity to use fatty acids either for generation of membrane lipids or of metabolic energy. Our lipidome contains several thousands of lipids, most of which contain fatty acids. Accordingly, the metabolism of fatty acids is extremely complex, and it is amazingly fast – a labeled fatty acid is found in hundreds of different compounds after five minutes of metabolism. Understanding this complexity and its pathological deviations needs experimental tools that offer high sensitivity and time resolution.
Tracing needs a tracer that is as similar as possible to the target molecule but nonetheless reliably distinguishable. Our tracers are alkynes - fatty acids and other lipids that contain a terminal triple bond. We feed them to biological systems in which they are metabolized, and then we collect and analyze the alkyne-containing (= labeled) lipids. For that, we have developed two technologies:
This is the new approach published in 2019 (Thiele et al. 2019). Extracted alkyne lipids are reacted with the reporter C171.
C171 is optimized for improved detection by mass spectrometry. The positive charge strongly improves ionization, and the trialkylammonium group shows predictable fragmentation in tandem MS2.
Key advantages are:
- Perfect specificity: labeled species are reliably discriminated from unlabeled species even if present in trace amounts
- Strongly increased sensitivity: improved ionization results in improved sensitivity, typically by 5 – 50-fold.
- Absolut quantification by addition of internal standards
Typical performance (75000 hepatocytes per sample):
- Upon a 5 min labeling, identification of 150 – 250 labeled species
- Upon a 1 h labeling, identification of up to 1000 labeled species
- 15 – 20 lipid classes, 10 with absolute quantification
Single cell analysis:
- Hepatocytes labeled for 3 h
- Single cells by limiting dilution
- 60 – 100 labeled species with absolute quantification
A set of deuterated versions of the reagent, the C175 reagents, enables a multiplexed version of the procedure
Additional advantages of multiplexing:
- Four fold increased sample turnover
- Improved sample-to-sample comparison by elimination of stochastic variation
Multiplexed and single cell tracing of lipid metabolism: A step by step protocol. Nature Protocol Exchange
And the Behind the Paper blog at Nature’s Protocol and Methods Community
By reaction with a fluorogenic coumarin dye, labeled lipids become fluorescent. After separation by TLC, the labeled spots can be identified by co-migrating external standards and quantified relative to each other. Absolute quantification is possible but not as reliable as in mass spectrometry.
- Easily accessible
- Parallel sample processing with high throughput
This method was originally published in 2012 (Thiele et al. 2012) and has since then been used in numerous publications:
Hepatic synthesis of triacylglycerols containing medium-chain fatty acids is dominated by diacylglycerol acyltransferase 1 and efficiently inhibited by etomoxir. Wunderling K, Leopold C, Jamitzky I, Yaghmour M, Zink F, Kratky D, Thiele C. Mol Metab. 2021 Mar;45:101150. doi: 10.1016/j.molmet.2020.101150.
Lipid-Droplet Formation Drives Pathogenic Group 2 Innate Lymphoid Cells in Airway Inflammation. Karagiannis F, Masouleh SK, Wunderling K, Surendar J, Schmitt V, Kazakov A, Michla M, Hölzel M, Thiele C, Wilhelm C. Immunity. 2020 Apr 14;52(4):620-634.e6. doi: 10.1016/j.immuni.2020.03.003.
Salo, Veijo T.; Li, Shiqian; Vihinen, Helena; Hölttä-Vuori, Maarit; Szkalisity, Abel; Horvath, Peter et al. (2019): Seipin Facilitates Triglyceride Flow to Lipid Droplet and Counteracts Droplet Ripening via Endoplasmic Reticulum Contact. In Dev. Cell 50 (4), 478-493.e9. DOI: 10.1016/j.devcel.2019.05.016.
Luukkonen, Panu K.; Nick, Auli; Hölttä-Vuori, Maarit; Thiele, Christoph; Isokuortti, Elina; Lallukka-Brück, Susanna et al. (2019): Human PNPLA3-I148M variant increases hepatic retention of polyunsaturated fatty acids. In JCI insight 4 (16). DOI: 10.1172/jci.insight.127902.
Segerer, Gabriela; Engelmann, Daria; Kaestner, Alexandra; Trötzmüller, Martin; Köfeler, Harald; Stigloher, Christian et al. (2018): A phosphoglycolate phosphatase/AUM-dependent link between triacylglycerol turnover and epidermal growth factor signaling. In Biochimica et biophysica acta. Molecular and cell biology of lipids 1863 (6), pp. 584–594. DOI: 10.1016/j.bbalip.2018.03.002.
Hofmann, Kristina; Lamberz, Christian; Piotrowitz, Kira; Offermann, Nina; But, Diana; Scheller, Anja et al. (2017): Tanycytes and a differential fatty acid metabolism in the hypothalamus. In Glia 65 (2), pp. 231–249. DOI: 10.1002/glia.23088.
Alecu, Irina; Tedeschi, Andrea; Behler, Natascha; Wunderling, Klaus; Lamberz, Christian; Lauterbach, Mario A. R. et al. (2017): Localization of 1-deoxysphingolipids to mitochondria induces mitochondrial dysfunction. In Journal of lipid research 58 (1), pp. 42–59. DOI: 10.1194/jlr.M068676.
Salo, Veijo T.; Belevich, Ilya; Li, Shiqian; Karhinen, Leena; Vihinen, Helena; Vigouroux, Corinne et al. (2016): Seipin regulates ER-lipid droplet contacts and cargo delivery. In The EMBO journal 35 (24), pp. 2699–2716. DOI: 10.15252/embj.201695170.
Merklinger, Elisa; Schloetel, Jan-Gero; Spitta, Luis; Thiele, Christoph; Lang, Thorsten (2016): No Evidence for Spontaneous Lipid Transfer at ER-PM Membrane Contact Sites. In The Journal of membrane biology 249 (1-2), pp. 41–56. DOI: 10.1007/s00232-015-9845-2.
Gaebler, Anne; Penno, Anke; Kuerschner, Lars; Thiele, Christoph (2016): A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. In Journal of lipid research 57 (10), pp. 1934–1947. DOI: 10.1194/jlr.D070565.
Wolf, Monika Julia; Adili, Arlind; Piotrowitz, Kira; Abdullah, Zeinab; Boege, Yannick; Stemmer, Kerstin et al. (2014): Metabolic activation of intrahepatic CD8+ T cells and NKT cells causes nonalcoholic steatohepatitis and liver cancer via cross-talk with hepatocytes. In Cancer cell 26 (4), pp. 549–564. DOI: 10.1016/j.ccell.2014.09.003.
Schneider, Christoph; Nobs, Samuel P.; Kurrer, Michael; Rehrauer, Hubert; Thiele, Christoph; Kopf, Manfred (2014): Induction of the nuclear receptor PPAR-γ by the cytokine GM-CSF is critical for the differentiation of fetal monocytes into alveolar macrophages. In Nature immunology 15 (11), pp. 1026–1037. DOI: 10.1038/ni.3005.
Moessinger, Christine; Klizaite, Kristina; Steinhagen, Almut; Philippou-Massier, Julia; Shevchenko, Andrej; Hoch, Michael et al. (2014): Two different pathways of phosphatidylcholine synthesis, the Kennedy Pathway and the Lands Cycle, differentially regulate cellular triacylglycerol storage. In BMC cell biology 15, p. 43. DOI: 10.1186/s12860-014-0043-3.
Itoe, Maurice A.; Sampaio, Júlio L.; Cabal, Ghislain G.; Real, Eliana; Zuzarte-Luis, Vanessa; March, Sandra et al. (2014): Host cell phosphatidylcholine is a key mediator of malaria parasite survival during liver stage infection. In Cell host & microbe 16 (6), pp. 778–786. DOI: 10.1016/j.chom.2014.11.006.
Hofmann, Kristina; Thiele, Christoph; Schött, Hans-Frieder; Gaebler, Anne; Schoene, Mario; Kiver, Yuriy et al. (2014): A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. In Journal of lipid research 55 (3), pp. 583–591. DOI: 10.1194/jlr.D044727.
Gaebler, Anne; Milan, Robin; Straub, Leon; Hoelper, Dominik; Kuerschner, Lars; Thiele, Christoph (2013): Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays. In Journal of lipid research 54 (8), pp. 2282–2290. DOI: 10.1194/jlr.D038653.
Kuerschner, Lars; Richter, Doris; Hannibal-Bach, Hans Kristian; Gaebler, Anne; Shevchenko, Andrej; Ejsing, Christer S.; Thiele, Christoph (2012): Exogenous ether lipids predominantly target mitochondria. In PloS one 7 (2), e31342. DOI: 10.1371/journal.pone.0031342.
Thiele, Christoph; Papan, Cyrus; Hoelper, Dominik; Kusserow, Kalina; Gaebler, Anne; Schoene, Mario et al. (2012): Tracing fatty acid metabolism by click chemistry. In ACS chemical biology 7 (12), pp. 2004–2011. DOI: 10.1021/cb300414v.